Journal: Oncology Letters

Article Title: MKP-1 overexpression is associated with chemoresistance in bladder cancer via the MAPK pathway

doi: 10.3892/ol.2020.11741

Figure Lengend Snippet: MKP-1 and MAPK expression in RT112 cells transfected with NC and MKP-1 siRNA. (A) Relative MKP-1 expression in the siNC and siMKP-1 groups was examined using reverse transcription-quantitative PCR. **P<0.01. (B) Representative microscopic images of siNC and siMKP-1 treated cells captured in both 2D and 3D environments under a phase contrast microscope. (C) MKP-1 expression of the siNC and siMKP-1 groups, as determined via western blotting. GAPDH was used as the internal control. (D) Phosphorylated and total ERK1/2 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (E) Phosphorylated and total p38 protein expressions of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. (F) Phosphorylated and total JNK protein expression levels of the siNC and siMKP-1 group, as determined via western blotting. GAPDH was used as the internal control. MKP-1, mitogen activated protein kinase phosphatase-1; NC, negative control; siMKP-1, MKP-1 small interfering RNA; siNC, small interfering negative control.

Article Snippet: 3D Cell Culture Gel (cat. no. P720M-10) was purchased from Col-Tgel Med ( http://www.101bio.com/P720_3D_cell_culture_gel.php ).

Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction, Microscopy, Western Blot, Negative Control, Small Interfering RNA

Journal: Stem Cell Reports

Article Title: A 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production

doi: 10.1016/j.stemcr.2014.03.012

Figure Lengend Snippet: 3D Sphere Culture of Human Pluripotent Stem Cells (A) Complete inhibition of hPSC sphere sedimentation by low-acyl GG at 0.015%. KhES-1 spheres on day 4 were suspended in the culture medium with various concentrations of GG and observed after 16 hr. The scale bar represents 5 mm. (B) Fold increase in KhES-1 cell number in culture medium with or without GG in different culture vessels. The average fold increase from independent experiments indicates the cell number increase from days 0 to 5, and the error bars indicate the SD (n = 10 for tube-shaped bags and n = 4 for others). Significance calculations were performed using the Student’s t test. (C) A tube-shaped culturing bag made of a gas-permeable membrane (left) and polystyrene tubes (15 and 5 ml). (D) Morphologies of KhES-1 spheres on day 5 in the 3D sphere culture medium with 0.020% GG. The scale bar represents 200 μm. (E) Comparison of KhES-1 cell expansion rates in the 3D sphere culture using tube-shaped gas-permeable bags or culture plates. The graph shows exemplary expansion rate plotting. Average slopes and SD in the semilogarithmical plotting were obtained by calculating exponential trend lines from three independent experiments and indicated in each graph. See also Figure S6 and .

Article Snippet: The 3D sphere culture medium was prepared at Nissan Chemical Industries as follows.

Techniques: Inhibition, Sedimentation, Membrane, Comparison

Journal: Stem Cell Reports

Article Title: A 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production

doi: 10.1016/j.stemcr.2014.03.012

Figure Lengend Snippet: Proof of Principle 3D hPSC Sphere Culture (A) A trial for larger-scale sphere culture by using 200 ml gas-permeable membrane bags. The scale bar represents 5 cm. (B) Comparison of cell yield calculated from the average cell number obtained in the 3D sphere or adherent culture using KhES-1 cell line. (C) Morphology of KhES-1 spheres cultured for 5 days in a 200 ml bag. The scale bar represents 200 μm.

Article Snippet: The 3D sphere culture medium was prepared at Nissan Chemical Industries as follows.

Techniques: Membrane, Comparison, Cell Culture